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User vs. Software-Dependent Variability of ELISPOT Counts Obtained from ten Different Laboratories

Published 06 May 2014 | By CTL Laboratories

In ELISPOT assays, cytokine spots produced by antigen-specific T cells show a broad spectrum of sizes and densities over variable background. Therefore, even experienced investigators are likely to come up with different spot counts when subjectively judging the minimal spot size/density to be counted and the maximal spot size for the cut off between singlecell-derived spots vs. those created by cell clusters. This study aims to find out whether statistics-based automated gating can harmonize spot counts obtained in different laboratories.

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User vs. Software-Dependent Variability of ELISPOT Counts Obtained from ten Different Laboratories