Contract Research & Services
Clinical Trials

Monitoring of Cell Mediated Immunity Focusing on T and B Cells

By CTL Laboratories

ELISPOT assays are the Ideal readout system for cytokine-based immune monitoring and standardisation strategies.


ELISPOT assays, and the related multiplex FluoroSpot technology, are ideal for measurement of per-cell antigen-specific cytokine production and direct ex vivo cytotoxicity assays. T cell functional avidity studies and epitope mapping, and B cell ELISPOT assays for determining the class or subclass of antibody-secreting cells, are just a few areas where ELISPOT and FluoroSpot are used.

We have been chosen repeatedly to serve as a reference laboratory for ELISPOT, including the Immune Tolerance Network and the Sabin Vaccine Institute's Cancer Vaccine Consortium. CTL has been awarded multiple IDIQ contracts by the U.S. National Institute of Health (NIH) for development and validation of human and animal models of infectious diseases, as well as for biodefense applications.

Furthermore, we have optimized, qualified, and validated test methods for our clients in various test systems: human, mouse, non-human primates, and pig, among others. This experience has provided CTL a wealth of expertise encompassing a wide range of assay types (ELISPOT, FluoroSpot, ELISA, multiplex Cytokine Bead Arrays (CBA), Flow Cytometry for cell phenotyping and multimer readouts etc., and BrdU proliferation assays) in both regulated and non-regulated studies.

CTL has recently developed novel ELISPOT and FluoroSpot assays for the analysis of antigen-specific B cell antibody production. We are now able to detect and analyze each IgG subclass produced by stimulated B cells simultaneously in the same assay well. Likewise, combinations of up to 4 antibody classes can be detected simultaneously, enabling large-scale, high-throughput screening providing a wealth of data using a fraction of the cell material.

CTL Laboratories has onsite facilities for processing whole blood, PBMC separation, and cryo-preservation of lymphocytes for subsequent functional assay testing. This allows us to act as a central processing facility for multisite studies. We are fully-equipped to handle the logistics of collection, processing, cataloging, and cryo-storage of samples from multiple clinical collection sites.

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